Wheat pathogenesis-related proteins of class 4 have ribonuclease activity

FEBS Lett. 2004 Sep 24;575(1-3):71-6. doi: 10.1016/j.febslet.2004.07.091.

Abstract

We have demonstrated that wheatwin1, a wheat pathogenesis-related protein of class 4 (PR4), has ribonuclease activity. Both native and recombinant proteins hydrolyse RNA from wheat coleoptils and have antifungal activity. Sepharose-bound wheatwin1 is able to interact with either wheat or Fusarium culmorum RNA. 3D modelling studies showed that, like ribonucleases A and T1, the action mechanism should involve two His residues, an Arg residue and an Asp residue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Fusarium / cytology
  • Fusarium / genetics
  • Fusarium / pathogenicity
  • Molecular Sequence Data
  • Molecular Structure
  • Plant Proteins / classification
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • RNA, Viral / metabolism
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Ribonucleases / classification
  • Ribonucleases / genetics
  • Ribonucleases / metabolism*
  • Spores, Fungal / metabolism
  • Triticum / enzymology*
  • Triticum / microbiology*

Substances

  • Plant Proteins
  • RNA, Viral
  • Recombinant Fusion Proteins
  • Win-1 proteins, plant
  • Ribonucleases