Melatonin application reduces fluoride uptake and toxicity in rice seedlings by altering abscisic acid, gibberellin, auxin and antioxidant homeostasis

The manuscript presents an elaborate report on the ameliorative effects of exogenous melatonin in soil-grown seedlings of the rice variety, IR-64 subjected to prolonged fluoride stress. Exogenous melatonin stimulated the physiological growth of the stressed seedlings by triggering high accumulation of gibberellic acid (GA) and melatonin via up regulation of the biosynthetic genes like GA3ox, TDC, SNAT and ASMT. The endogenous abscisic acid (ABA) content increased via induction of NCED3 and suppression of ABA8ox1. However, the ABA-dependent genes like TRAB1, WRKY71 and OSBZ8 were down regulated in presence of high endogenous GA and melatonin. High melatonin level led to low indole-3-acetic acid accumulation in the treated seedlings during fluoride stress. Melatonin significantly decreased fluoride bioaccumulation by suppressing its uptake via CLC1 and CLC2, and also restored P-H⁺/ATPase expression. The damage indices like chlorosis (accompanied by low RuBisCo), malondialdehyde, electrolyte leakage, methylglyoxal (detoxified by glyoxalase II) and protein carbonylation were greatly reduced. Increased proline synthesis, activation of the ascorbate-glutathione cycle and enhanced activity of glutathione peroxidase, catalase and guaiacol peroxidase led to low ROS accumulation and localization in the melatonin-treated plants exposed to stress. Overall, melatonin treatment alleviated fluoride-mediated injuries by restricting fluoride uptake, refining the defence machinery and altering the phytohormone homeostasis.