In vivo stimulatory effect of macrophage colony-stimulating factor on the number of stroma-initiating cells

J Cell Physiol. 1999 Feb;178(2):267-73. doi: 10.1002/(SICI)1097-4652(199902)178:2<267::AID-JCP16>3.0.CO;2-8.

Abstract

The in vivo effect of human macrophage colony-stimulating factor (M-CSF) on the number of cells that formed stromal colonies in an in vitro culture system (stroma-initiating cells; SICs) was investigated. We found that the number of SICs in the femurs of C57BL/6 mice was significantly increased by the treatment with M-CSF. We also found that the SICs were resistant to at least three different chemotherapeutic reagents, 5-fluorouracil (5-FU), cytarabine, and cyclophosphamide, because the femoral cells of mice treated with these reagents contained higher numbers of SICs than those of untreated mice. M-CSF treatment also increased the number of SICs of the reagent-pretreated mice. The SICs detected in our culture system were present only in Mac-1(-)CD45(-) cells, and the M-CSF treatment of 5-FU-pretreated mice actually increased the number of Mac-1(-)CD45(-) SICs. The Mac-1(-)CD45(-) SICs collected from mice that were pretreated with 5-FU and then treated with M-CSF formed stromal colonies under in vitro culture conditions that did not contain M-CSF but did contain a high concentration of fetal calf serum. This result suggested that SICs collected following the treatment procedure did not necessarily require the presence of M-CSF for their in vitro proliferation. Our study indicated that M-CSF has the ability to increase the number of progenitor or precursor cells for bone marrow stromal cells in vivo system.

MeSH terms

  • Animals
  • Antineoplastic Agents / pharmacology
  • Colony-Forming Units Assay
  • Cyclophosphamide / pharmacology
  • Cytarabine / pharmacology
  • Drug Resistance
  • Fluorouracil / pharmacology
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / drug effects*
  • Hematopoietic Stem Cells / immunology
  • Humans
  • Leukocyte Common Antigens / metabolism
  • Macrophage Colony-Stimulating Factor / pharmacology*
  • Macrophage-1 Antigen / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Stromal Cells / cytology*
  • Stromal Cells / drug effects*
  • Stromal Cells / immunology

Substances

  • Antineoplastic Agents
  • Macrophage-1 Antigen
  • Cytarabine
  • Macrophage Colony-Stimulating Factor
  • Cyclophosphamide
  • Leukocyte Common Antigens
  • Fluorouracil