Ki-67 immunostaining and stereologic estimation of nuclear volume in melanocytic skin tumors

Anal Quant Cytol Histol. 1999 Feb;21(1):42-6.

Abstract

Objective: To investigate the proliferative activity and mean nuclear volume (MNV) of melanocytic skin tumors.

Study design: Proliferative activity, assessed by immunostaining for the Ki-67 monoclonal antibody (reactive with all actively cycling cells), and MNV, estimated by means of a stereologic method, were determined in 60 cutaneous melanocytic tumors, including 28 primary malignant melanomas (PMM), 13 compound nevi (CN), 11 dysplastic nevi and 8 metastatic malignant melanomas.

Results: Both MNV and Ki-67 expression differed significantly between CN and other melanocytic tumors and showed a good correlation with Clark's level (a well-established prognostic parameter in PMM).

Conclusion: The association of proliferative activity and quantitative nuclear features may be helpful in the interpretation of the degree of malignancy in melanocytic skin tumors.

MeSH terms

  • Cell Division
  • Humans
  • Image Processing, Computer-Assisted
  • Immunohistochemistry
  • Karyometry
  • Ki-67 Antigen / metabolism*
  • Melanoma / pathology*
  • Microscopy, Video
  • Skin Neoplasms / pathology*

Substances

  • Ki-67 Antigen