Abstract
Segregation Distorter (SD) in Drosophila melanogaster is a naturally occurring meiotic drive system in which the SD chromosome is transmitted from SD/SD+ males in vast excess over its homolog owing to the induced dysfunction of SD+-bearing spermatids. The Sd locus is the key distorting gene responsible for this phenotype. A genomic fragment from the Sd region conferred full distorting activity when introduced into the appropriate genetic background by germline transformation. The only functional product encoded by this fragment is a truncated version of the RanGAP nuclear transport protein. These results demonstrate that this mutant RanGAP is the functional Sd product.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Carrier Proteins / chemistry
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Carrier Proteins / genetics*
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Carrier Proteins / physiology
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Cell Nucleus / metabolism
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Crosses, Genetic
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DNA, Complementary
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Drosophila Proteins*
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Drosophila melanogaster / genetics*
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Drosophila melanogaster / physiology
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Female
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GTPase-Activating Proteins*
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Gene Duplication
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Gene Expression
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Genes, Insect*
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Male
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Meiosis*
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Nuclear Proteins / chemistry
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Nuclear Proteins / genetics*
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Nuclear Proteins / physiology
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RNA, Messenger / genetics
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Spermatids / physiology
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Sulfotransferases / chemistry
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Sulfotransferases / genetics
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Transcription, Genetic
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Transformation, Genetic
Substances
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Carrier Proteins
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DNA, Complementary
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Drosophila Proteins
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GTPase-Activating Proteins
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Nuclear Proteins
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RNA, Messenger
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RanGAP protein, Drosophila
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Sulfotransferases
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heparan-sulfate 2-sulfotransferase