Abstract
Restriction fragment length polymorphism and hybridization of DNA extracted from Mycobacterium tuberculosis, nontuberculous mycobacteria, and nonmycobacterial species with a probe derived from IS6110 confirmed that IS6110 was specific to M. tuberculosis complex. In addition, DNA amplification with IS6110-specific primers yielded a 181-bp fragment only in DNA from M. tuberculosis complex isolates.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteriological Techniques
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Base Sequence
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DNA Fingerprinting / methods*
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DNA Primers / genetics
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DNA Transposable Elements / genetics*
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DNA, Bacterial / genetics
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Gene Amplification
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Humans
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Mycobacterium tuberculosis / genetics*
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Mycobacterium tuberculosis / isolation & purification*
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Polymorphism, Restriction Fragment Length
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Species Specificity
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Tuberculosis, Pulmonary / diagnosis*
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Tuberculosis, Pulmonary / microbiology
Substances
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DNA Primers
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DNA Transposable Elements
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DNA, Bacterial