Abstract
A permanent cell line, U-BLC1, was established from a primary transitional-cell carcinoma, TCC, of the urinary bladder. Karyotype analysis showed the line to be highly aberrant, with a near-triploid chromosome number of 68 to 73. Comparative genomic hybridization revealed some distinct differences between the primary tumor and the established cell line. Karyotype analysis showed 3 marker chromosomes with homogeneously staining regions, HSRs, in the cell line. The HSRs were isolated by microdissection and the microdissection probes were hybridized to normal metaphase chromosomes. The HSRs contain sequences known to be frequently involved in amplification in transitional-cell carcinoma of the bladder, 6p22, 7p11-p12, 9p23-pter, and one region not yet reported to be amplified in primary TCC of the bladder, 1p31-p32. A candidate-gene approach showed that in the region 7p11-p12 the EGFR locus is amplified and highly expressed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aged
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Aged, 80 and over
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Aneuploidy
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Blotting, Northern
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Carcinoma, Transitional Cell / genetics*
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Carcinoma, Transitional Cell / pathology
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Chromosomes, Human / ultrastructure*
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Chromosomes, Human, Pair 1 / genetics
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Chromosomes, Human, Pair 1 / ultrastructure
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Chromosomes, Human, Pair 6 / genetics
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Chromosomes, Human, Pair 6 / ultrastructure
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Chromosomes, Human, Pair 7 / genetics
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Chromosomes, Human, Pair 7 / ultrastructure
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Chromosomes, Human, Pair 9 / genetics
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Chromosomes, Human, Pair 9 / ultrastructure
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ErbB Receptors / biosynthesis
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ErbB Receptors / genetics
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Female
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Gene Amplification
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Humans
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In Situ Hybridization, Fluorescence
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Karyotyping
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Neoplasm Proteins / biosynthesis
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Neoplasm Proteins / genetics
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Polymerase Chain Reaction
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Translocation, Genetic / genetics
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Tumor Cells, Cultured / ultrastructure
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Urinary Bladder Neoplasms / genetics*
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Urinary Bladder Neoplasms / pathology
Substances
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Neoplasm Proteins
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ErbB Receptors