Abstract
Fungal-specific primers targeted for highly conserved genomic nucleic acid sequences were used in a polymerase chain reaction (PCR) to amplify DNA from lobomycosis lesions in a bottlenose dolphin. Sequence alignments of this DNA possessed high homology to fungal ribosomal DNA sequences found in the genus Cladosporium. When used for in situ hybridization, the riboprobe transcribed from a cloned PCR-generated fragment bound to Loboa loboi cells. These results support the hypothesis that L. loboi in dolphin tissue is a fungus.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Cladosporium / genetics
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DNA, Fungal / genetics
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DNA, Fungal / isolation & purification
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DNA, Ribosomal / genetics
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DNA, Ribosomal / isolation & purification*
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Dermatomycoses / microbiology
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Dermatomycoses / veterinary*
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Dolphins / microbiology*
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Entomophthorales*
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Molecular Sequence Data
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Sequence Alignment
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Sequence Homology, Nucleic Acid
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Skin / microbiology*
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Zygomycosis / microbiology
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Zygomycosis / veterinary*
Substances
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DNA, Fungal
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DNA, Ribosomal