Abstract
The putative metal-transporter protein PsaA of Streptococcus pneumoniae is of potential interest both as a vaccine and also as a drug target. The overexpression of the protein in E. coli, and its subsequent purification and crystallization are described. The crystals are rectangular rods and diffract to beyond 2.7 A resolution. The crystal space group is P212121 with unit-cell dimensions a = 59.9, b = 66.5 and c = 69.9 A.
MeSH terms
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Adhesins, Bacterial
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Bacterial Proteins*
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Carrier Proteins / biosynthesis
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Carrier Proteins / chemistry*
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Carrier Proteins / genetics
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Carrier Proteins / isolation & purification
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Cloning, Molecular
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Crystallization
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Crystallography, X-Ray
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Escherichia coli
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Lipoproteins / biosynthesis
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Lipoproteins / chemistry*
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Lipoproteins / genetics
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Lipoproteins / isolation & purification
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Membrane Transport Proteins*
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / isolation & purification
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Streptococcus pneumoniae / chemistry*
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Streptococcus pneumoniae / genetics
Substances
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Adhesins, Bacterial
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Bacterial Proteins
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Carrier Proteins
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Lipoproteins
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Membrane Transport Proteins
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PsaA protein, Streptococcus
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Recombinant Fusion Proteins