Frameshift mutations in TGFbetaRII, IGFIIR, BAX, hMSH3 and hMSH6 are absent in lung cancers

K Gotoh; Y Yatabe; T Sugiura; K Takagi; M Ogawa; T Takahashi; T Mitsudomi

doi:10.1093/carcin/20.3.499

Frameshift mutations in TGFbetaRII, IGFIIR, BAX, hMSH3 and hMSH6 are absent in lung cancers

Carcinogenesis. 1999 Mar;20(3):499-502. doi: 10.1093/carcin/20.3.499.

Authors

K Gotoh¹, Y Yatabe, T Sugiura, K Takagi, M Ogawa, T Takahashi, T Mitsudomi

Affiliation

¹ Department of Internal Medicine and Pulmonary Medicine, Aichi Cancer Center Hospital, Nagoya University School of Medicine, Japan.

PMID: 10190568
DOI: 10.1093/carcin/20.3.499

Abstract

A genome-wide instability at simple repeat sequences characterizes gastrointestinal and endometrial cancers of the microsatellite mutator phenotype (MMP). The genes encoding transforming growth factor-beta receptor type II (TGFbetaRII), insulin-like growth factor II receptor (IGFIIR), Bcl-2 associated X protein (BAX), hMSH3 and hMSH6 have simple repeat sequences in their coding regions. Consequently, mutations in the single repeat sequences in these genes provide one major route for carcinogenesis in these cancers. We examined 43 non-small cell lung carcinomas and 16 small cell carcinomas for frameshift mutations in simple repeat sequences of TGFbetaRII, IGFIIR, BAX, hMSH3 and hMSH6. In addition, MMP was assessed using a primer set for BAT-26. None of 59 lung cancers exhibited frameshift mutations or MMP. It is concluded that somatic frameshift mutations in these genes and MMP do not constitute important mechanisms in lung carcinogenesis. The possibility of some sort of genetic instability undetectable as a form of MMP cannot be precluded.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Carcinoma, Non-Small-Cell Lung / genetics
Carcinoma, Small Cell / genetics
DNA Primers
DNA-Binding Proteins / genetics*
Frameshift Mutation*
Humans
Lung Neoplasms / genetics*
Multidrug Resistance-Associated Proteins*
MutS Homolog 3 Protein
Polymerase Chain Reaction
Protein Serine-Threonine Kinases
Proto-Oncogene Proteins / genetics
Proto-Oncogene Proteins c-bcl-2*
Receptor, Transforming Growth Factor-beta Type II
Receptors, Somatomedin / genetics
Receptors, Transforming Growth Factor beta / genetics
bcl-2-Associated X Protein

Substances

BAX protein, human
DNA Primers
DNA-Binding Proteins
G-T mismatch-binding protein
MSH3 protein, human
Multidrug Resistance-Associated Proteins
MutS Homolog 3 Protein
Proto-Oncogene Proteins
Proto-Oncogene Proteins c-bcl-2
Receptors, Somatomedin
Receptors, Transforming Growth Factor beta
bcl-2-Associated X Protein
Protein Serine-Threonine Kinases
Receptor, Transforming Growth Factor-beta Type II
multidrug resistance-associated protein 1