Abstract
We have recently identified the HMG box transcription factor Sox-13 and described its expression during murine embryogenesis. Here we describe the structure of the murine Sox-13 gene. This gene spans approximately 12 kb and consists of 13 exons. The HMG domain is encoded by exons XI and XII, separated by an intron that is conserved among Sox-5, Sox-13, and Sox-17. A single major transcription initiation site was identified. Deletion analysis of the 3-kb promoter region revealed a 400-bp fragment driving transcription of a luciferase reporter in a Sox-13-expressing cell line. To determine the chromosomal localization of the human gene, a human SOX13 cDNA was isolated with 75% homology to the mouse Sox-13. FISH analysis mapped the human SOX13 gene to chromosome 1 band q32.
Copyright 1999 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Autoantigens*
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Base Sequence
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Blotting, Northern
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Cell Line
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Chromosome Mapping
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Chromosomes, Human, Pair 1 / genetics
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DNA / chemistry
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DNA / genetics
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Exons
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Gene Expression Regulation
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High Mobility Group Proteins / genetics*
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Humans
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In Situ Hybridization, Fluorescence
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Introns
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Luciferases / genetics
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Luciferases / metabolism
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Mice
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Molecular Sequence Data
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Promoter Regions, Genetic
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RNA / genetics
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RNA / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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SOXD Transcription Factors
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Sequence Analysis, DNA
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Tissue Distribution
Substances
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Autoantigens
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High Mobility Group Proteins
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Recombinant Fusion Proteins
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SOX13 protein, human
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SOXD Transcription Factors
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Sox13 protein, mouse
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RNA
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DNA
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Luciferases
Associated data
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GENBANK/AF057702
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GENBANK/AF083105