Cutting edge: recruitment of the CD19/CD21 coreceptor to B cell antigen receptor is required for antigen-mediated expression of Bcl-2 by resting and cycling hen egg lysozyme transgenic B cells

J Immunol. 1999 Apr 15;162(8):4377-80.

Abstract

Recruitment of the CD19/CD21 coreceptor is thought to lower the threshold for effective signaling through the B cell Ag receptor. We provide evidence supporting a second role for coreceptor recruitment, and that is to enhance the survival/proliferative potential of the responding B cells. We show that B cell Ag receptor signaling in the absence of coreceptor recruitment induces cellular accumulation of the anti-apoptotic protein Bcl-xL, whereas CD19-mediated signals are required for Bcl-2 accumulation. The expression of both anti-apoptotic proteins correlates with the enhanced responsiveness of both resting and cycling B cells to growth-promoting signals delivered through CD40. These results provide further evidence for the necessity of coreceptor recruitment during Ag-dependent B cell activation and indicate that Ags derived from inflammatory sites function as better thymus-dependent Ags than their counterparts not coated with complement fragments.

MeSH terms

  • Animals
  • Antibodies, Anti-Idiotypic / physiology
  • Antigens, CD19 / metabolism*
  • Antigens, CD19 / physiology
  • B-Lymphocyte Subsets / immunology
  • B-Lymphocyte Subsets / metabolism*
  • Cell Cycle / genetics
  • Cell Cycle / immunology
  • Cells, Cultured
  • Female
  • Immunoglobulin mu-Chains / immunology
  • Interphase / genetics
  • Interphase / immunology
  • Lymphocyte Activation
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Muramidase / genetics*
  • Muramidase / immunology*
  • Proto-Oncogene Proteins c-bcl-2 / biosynthesis*
  • Receptor Aggregation / immunology
  • Receptors, Antigen, B-Cell / metabolism*
  • Receptors, Antigen, B-Cell / physiology
  • Receptors, Complement 3d / metabolism*
  • Receptors, Complement 3d / physiology
  • Signal Transduction / immunology
  • Transgenes / immunology*

Substances

  • Antibodies, Anti-Idiotypic
  • Antigens, CD19
  • Immunoglobulin mu-Chains
  • Proto-Oncogene Proteins c-bcl-2
  • Receptors, Antigen, B-Cell
  • Receptors, Complement 3d
  • Muramidase