Abstract
Human angiogenin was genetically engineered and contained the E. coli Omp A signal sequence for secreting soluble angiogenin to the periplasm under tac promoter control. The angiogenin sequence was encoded in a single gene and expressed as a 14.4 kilodalton soluble protein in E. coli. It was purified by CM-Sepharose ion-exchange chromatography and by a heparin-Sepharose affinity chromatography procedure. The biological activity of angiogenin was established by its ability to inhibit mRNA-dependent rabbit reticulocyte cell-free translation.
MeSH terms
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Bacterial Outer Membrane Proteins / genetics*
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Chromatography, Affinity
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Chromatography, Agarose
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Escherichia coli / genetics*
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Gene Expression
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Genetic Engineering
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Humans
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Plasmids / genetics
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Promoter Regions, Genetic
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Protein Biosynthesis*
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Proteins / genetics
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Proteins / isolation & purification
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Recombinant Proteins / isolation & purification
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Reticulocytes / metabolism
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Ribonuclease, Pancreatic*
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Solubility
Substances
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Bacterial Outer Membrane Proteins
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Proteins
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Recombinant Proteins
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OMPA outer membrane proteins
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angiogenin
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Ribonuclease, Pancreatic