Isolation and sequence of the GCR3 homologue from Candida albicans by complementation of (delta)gcr3 strain of Saccharomyces cerevisiae

H Uemura; K Nakamoto; S Sugioka; M Tadenuma

doi:10.1002/(SICI)1097-0061(19990315)15:4<323::AID-YEA363>3.0.CO;2-L

Isolation and sequence of the GCR3 homologue from Candida albicans by complementation of (delta)gcr3 strain of Saccharomyces cerevisiae

Yeast. 1999 Mar 15;15(4):323-7. doi: 10.1002/(SICI)1097-0061(19990315)15:4<323::AID-YEA363>3.0.CO;2-L.

Authors

H Uemura¹, K Nakamoto, S Sugioka, M Tadenuma

Affiliation

¹ Department of Molecular Biology, National Institute of Bioscience and Human-Technology, Tsukuba, Ibaraki, Japan. [email protected]

PMID: 10206191
DOI: 10.1002/(SICI)1097-0061(19990315)15:4<323::AID-YEA363>3.0.CO;2-L

Abstract

To study the function of GCR3, a gene involved in the expression of glycolytic genes in Saccharomyces cerevisiae, a Candida albicans gene which complements the growth defect of the (delta)gcr3 mutant was isolated. Transformants of this gene also recovered the glycolytic enzyme activities. Its DNA sequencing predicted an 886 amino acid protein with 30.4% identity to the Gcr3p of S. cerevisiae.

MeSH terms

Amino Acid Sequence
Base Sequence
Candida albicans / genetics*
Fungal Proteins / chemistry
Fungal Proteins / genetics*
Genetic Complementation Test
Molecular Sequence Data
Nuclear Proteins / chemistry
Nuclear Proteins / genetics*
Plasmids / genetics
RNA Cap-Binding Proteins
Restriction Mapping
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins*
Sequence Analysis, DNA
Sequence Homology, Amino Acid

Substances

Fungal Proteins
Nuclear Proteins
RNA Cap-Binding Proteins
STO1 protein, S cerevisiae
Saccharomyces cerevisiae Proteins

Associated data

GENBANK/AB015281