Abstract
The Sec23p-Sec24p complex is a component of COPII-coated vesicles that mediate protein transport from the endoplasmic reticulum in yeast. The mammalian hypothetical protein KIAA0079 (KIAA0079p) exhibits sequence similarity to yeast Sec24p. KIAA0079p was co-eluted with mammalian Sec23p on gel filtration. In vitro binding experiments revealed that the C-terminal region of KIAA0079p binds to the N-terminal region of mammalian Sec23p. Overexpression of KIAA0079p caused a defect in protein export from the endoplasmic reticulum. These results support the idea that KIAA0079p is a functional homologue of yeast Sec24p.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Binding Sites
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Biological Transport, Active
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Cell Line
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Chlorocebus aethiops
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Endoplasmic Reticulum / metabolism
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Fungal Proteins / chemistry
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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Humans
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In Vitro Techniques
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Membrane Proteins / chemistry
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Proteins / chemistry
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Proteins / isolation & purification
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Proteins / metabolism
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins*
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Transfection
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Vero Cells
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Vesicular Transport Proteins
Substances
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Fungal Proteins
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Membrane Proteins
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Proteins
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SEC23A protein, human
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SEC24 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Vesicular Transport Proteins