Abstract
The tetracycline resistance region of the 50-kb R-plasmid pTP10 from the clinical isolate Corynebacterium striatum M82B was analyzed in Corynebacterium glutamicum ATCC 13032 and confined to a 4.4-kb SphI-Sa/I DNA fragment. Nucleotide sequence analysis revealed two open reading frames, termed tetA and tetB, specifying proteins of 513 and 528 amino acids, respectively. The deduced amino acid sequences of tetAB displayed similarity to ATP-binding cassette transporters including StrV and StrW of Streptomyces glaucescens which are proposed to play a role in the export of streptomycin-like aminoglycosides. An antibiotic susceptibility screening in C. glutamicum showed that the tetAB genes confer resistance to tetracycline, oxytetracycline and to the structurally and functionally unrelated beta-lactam antibiotic oxacillin.
MeSH terms
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ATP-Binding Cassette Transporters / chemistry
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ATP-Binding Cassette Transporters / genetics*
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Amino Acid Sequence
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Anti-Bacterial Agents / pharmacology
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Antiporters / chemistry
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Antiporters / genetics*
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics*
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Corynebacterium / drug effects
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Corynebacterium / genetics*
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Corynebacterium Infections / microbiology
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Drug Resistance, Multiple / genetics*
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Genes, Bacterial
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Humans
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Oxacillin / pharmacology
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Oxytetracycline / pharmacology
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Penicillin Resistance / genetics
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Penicillins / pharmacology
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R Factors / genetics*
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Sequence Alignment
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Sequence Analysis, DNA
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Tetracycline / pharmacology
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Tetracycline Resistance / genetics
Substances
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ATP-Binding Cassette Transporters
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Anti-Bacterial Agents
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Antiporters
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Bacterial Proteins
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Penicillins
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TETP protein, Clostridium perfringens
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tetA protein, Bacteria
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Tetracycline
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Oxacillin
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Oxytetracycline