Abstract
Embryonic day 14 rat midbrain cultures were kept for 7 days in vitro and then intoxicated with radical donors iron and sodium-nitroprusside for 24 h. Tyrosine-hydroxylase positive neurons in cultures which were additionally treated with growth/differentiation factor-5 (GDF-5) survived to a significantly higher percentage as compared to sister cultures without factor supplementation. Since the degeneration of TH positive cells is a key feature in Parkinson's disease, GDF-5 might be a putative therapeutical agent for this disorder.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis / drug effects
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Bone Morphogenetic Proteins*
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Cell Survival / drug effects
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Cells, Cultured
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Dopamine / metabolism*
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Dose-Response Relationship, Drug
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Ferrous Compounds / toxicity
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Free Radicals / toxicity*
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Growth Differentiation Factor 5
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Growth Substances / pharmacology*
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Growth Substances / therapeutic use
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Lethal Dose 50
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Mesencephalon / cytology*
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Mesencephalon / embryology
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Neurons / cytology*
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Neurons / drug effects*
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Neurons / metabolism
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Nitric Oxide Donors / pharmacology
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Nitroprusside / pharmacology
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Parkinson Disease / drug therapy
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Rats
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Rats, Wistar
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Tyrosine 3-Monooxygenase / metabolism
Substances
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Bone Morphogenetic Proteins
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Ferrous Compounds
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Free Radicals
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Growth Differentiation Factor 5
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Growth Substances
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Nitric Oxide Donors
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Nitroprusside
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Tyrosine 3-Monooxygenase
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ferrous chloride
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Dopamine