The voltammetric behavior of human mammalian cells was studied by choosing human leukemia cells (HL60) and human erythroleukemia cells (HEL). The voltammetric response of the cells was found having relation with cell metabolic viability in culture course. For example, the fluctuations of peak currents of HL60 were parallel with the nutrients replenished or not, which can reflect cell health state; the voltammetric response of HL60 regulated by the anti-metabolic drug 5F-Uriacil (5F-U) in culture course behaved in a much decreased manner, by which a voltammetric method for evaluating cytotoxicity is proposed. In this paper, the relation between HEL cell metabolism and the activation of receptor Mpl by its ligand TPO was also studied. Moreover, the mechanism of cell voltammetric behavior was discussed.