Abstract
The thymus is colonized by circulating progenitor cells that differentiate into mature T cells under the influence of the thymic microenvironment. We report here the cloning and function of the avian thymocyte Ag ChT1, a member of the Ig superfamily with one V-like and one C2-like domain. ChT1-positive embryonic bone marrow cells coexpressing c-kit give rise to mature T cells upon intrathymic cell transfer. ChT1-specific Ab inhibits T cell differentiation in embryonic thymic organ cultures and in thymocyte precursor cocultures on stromal cells. Thus, we provide clear evidence that ChT1 is a novel Ag on early T cell progenitors that plays an important role in the early stages of T cell development.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Antigens, Surface / genetics
-
Base Sequence
-
Bone Marrow Transplantation
-
CD5 Antigens / genetics
-
CD5 Antigens / immunology*
-
Cell Differentiation
-
Chick Embryo
-
Chickens
-
Cloning, Molecular
-
DNA, Complementary / genetics
-
Hematopoietic Stem Cells / immunology
-
Immunoglobulins / genetics
-
Immunoglobulins / immunology*
-
Lymphoid Tissue / embryology
-
Lymphoid Tissue / immunology
-
Membrane Proteins / genetics
-
Molecular Sequence Data
-
Organ Culture Techniques
-
Proto-Oncogene Proteins c-kit / isolation & purification
-
Sequence Analysis, DNA
-
Sequence Homology, Amino Acid
-
T-Lymphocytes / cytology*
-
T-Lymphocytes / immunology*
-
Thymus Gland / cytology
-
Thymus Gland / immunology*
-
Thymus Gland / surgery
-
Tissue Distribution
Substances
-
Antigens, Surface
-
CD5 Antigens
-
DNA, Complementary
-
Immunoglobulins
-
Membrane Proteins
-
Proto-Oncogene Proteins c-kit
Associated data
-
GENBANK/Y14063
-
GENBANK/Y14064