Identification of multiple HIV-1 CTL epitopes presented by HLA-B*5101 molecules

Hum Immunol. 1999 Mar;60(3):177-86. doi: 10.1016/s0198-8859(98)00113-x.

Abstract

We attempted to identify and characterize HIV-1 CTL epitopes presented by HLA-B51 which is associated with a slow progression to AIDS. HLA-B*5101 stabilization assay showed that 33 out of 172 HIV-1 peptides carrying HLA-B*5101 anchor residues bound to HLA-B*5101. Seven peptides were suggested as HIV-1 CTL epitopes presented by HLA-B*5101 because the specific CTL was induced for these peptides in PBMC from three HIV-1 seropositive individuals carrying HLA-B51 by stimulation with HLA-B*5101 binding peptides. Analysis of these epitopes using the specific CTL clones confirmed that six of seven HIV-1 peptides are epitopes presented by HLA-B*5101. Three epitopes presented by HLA-B*5101 are highly conserved among the clade B strain, suggesting that the specific CTL for these epitopes might play an important role in recognition of HIV-1 infected cells. These epitopes will be useful to analyze CTL responses in HIV-1 infected individuals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigen Presentation*
  • Asian People
  • Clone Cells
  • Epitopes*
  • HIV Seropositivity / immunology*
  • HIV-1 / immunology*
  • HLA-B Antigens / immunology*
  • HLA-B51 Antigen
  • Humans
  • Oligopeptides / immunology
  • T-Lymphocytes, Cytotoxic / cytology
  • T-Lymphocytes, Cytotoxic / immunology*

Substances

  • Epitopes
  • HLA-B Antigens
  • HLA-B51 Antigen
  • Oligopeptides