Perturbation of RET signaling in the embryonic kidney

Dev Genet. 1999;24(3-4):263-72. doi: 10.1002/(SICI)1520-6408(1999)24:3/4<263::AID-DVG9>3.0.CO;2-D.

Abstract

We have used a RET-Ig fusion protein to disrupt signaling in the rat embryonic kidney development pathway. Treatment of embryonic kidney organ cultures with RET-Ig results in a decrease in branching of the ureteric bud and a down regulation in expression of the Wnt-11, Wnt-4, and ld genes. These data suggest that Wnt-11, Wnt-4, and ld function downstream of RET signaling in normal development. Expression of BMP-7, shh, and ptc were uneffected by RET-Ig treatment, implying that these genes are regulated independently of ret. We have also performed immunohistochemistry with a GFR alpha-1 specific polyclonal antisera to localize GFR alpha-1 protein expression in the developing kidney.

MeSH terms

  • Animals
  • Base Sequence
  • Bone Morphogenetic Protein 7
  • Bone Morphogenetic Proteins / genetics
  • DNA Primers / genetics
  • Drosophila Proteins*
  • Fetal Proteins / genetics
  • Formins
  • Gene Expression Regulation, Developmental
  • Glial Cell Line-Derived Neurotrophic Factor Receptors
  • Glycoproteins / genetics
  • Hedgehog Proteins
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization
  • Kidney / embryology*
  • Kidney / metabolism*
  • Membrane Proteins / genetics
  • Microfilament Proteins
  • Nuclear Proteins / genetics
  • Organ Culture Techniques
  • Patched Receptors
  • Proteins / genetics
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins c-ret
  • Rats
  • Receptor Protein-Tyrosine Kinases / genetics*
  • Receptors, Cell Surface
  • Recombinant Fusion Proteins / genetics
  • Signal Transduction
  • Trans-Activators*
  • Transforming Growth Factor beta*
  • Wnt Proteins
  • Wnt4 Protein

Substances

  • BMP7 protein, human
  • Bmp7 protein, rat
  • Bone Morphogenetic Protein 7
  • Bone Morphogenetic Proteins
  • DNA Primers
  • Drosophila Proteins
  • Fetal Proteins
  • Formins
  • GFRA1 protein, human
  • Gfra1 protein, rat
  • Glial Cell Line-Derived Neurotrophic Factor Receptors
  • Glycoproteins
  • Hedgehog Proteins
  • Membrane Proteins
  • Microfilament Proteins
  • Nuclear Proteins
  • Patched Receptors
  • Proteins
  • Proto-Oncogene Proteins
  • Receptors, Cell Surface
  • Recombinant Fusion Proteins
  • SHH protein, human
  • Trans-Activators
  • Transforming Growth Factor beta
  • WNT4 protein, human
  • Wnt Proteins
  • Wnt4 Protein
  • Wnt4 protein, rat
  • Proto-Oncogene Proteins c-ret
  • Receptor Protein-Tyrosine Kinases
  • Ret protein, Drosophila
  • Ret protein, rat