Presence of peptidase activities in different varieties of cheese

Lett Appl Microbiol. 1999 May;28(5):368-72. doi: 10.1046/j.1365-2672.1999.00541.x.

Abstract

Lactic acid bacteria (LAB) counts, PepX activity towards H-Phe-Pro-beta NA, and aminopeptidase activity towards H-Arg-beta NA. HCl, H-Lys-beta NA, H-Leu-beta NA, H-Pro-beta NA, H-Glu-beta NA derivatives have been evaluated in 32 commercial samples of cheese, one processed cheese, and one yoghurt. The presence of intracellular exo-peptidase activities in cheese extracts free from bacterial cells was detected, even after 1 year of ripening. An inverse ratio between the presence of viable lactic microflora and peptidase activity in the cheese extracts was observed. The importance of LAB starter exo-peptidases in the degradation of casein oligopeptides, and the key role of autolysis in the release of peptidases in the cheese, are discussed.

MeSH terms

  • Cheese / microbiology*
  • Colony Count, Microbial
  • Exopeptidases
  • Gram-Positive Bacteria / enzymology*
  • Peptide Hydrolases / isolation & purification*

Substances

  • Exopeptidases
  • Peptide Hydrolases