Abstract
The glutamyl-tRNA synthetase (gltX) gene from Pseudomonas aeruginosa was identified. A plasmid containing a 2.3-kb insert complemented the temperature-sensitive gltX mutation of Escherichia coli JP1449, and GltX activity was demonstrated. The inferred amino acid sequence of this gene showed 50.6% identity with GltX from Rhizobium meliloti.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Catalysis / drug effects
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Cloning, Molecular
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Conserved Sequence / genetics
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Genetic Complementation Test
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Glutamate-tRNA Ligase / chemistry
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Glutamate-tRNA Ligase / genetics*
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Glutamate-tRNA Ligase / isolation & purification
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Glutamate-tRNA Ligase / metabolism
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Molecular Sequence Data
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Mutation
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Phenanthrolines / pharmacology
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Pseudomonas aeruginosa / enzymology*
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Pseudomonas aeruginosa / genetics
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Sequence Alignment
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Sinorhizobium meliloti / enzymology
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Sinorhizobium meliloti / genetics
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Temperature
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Zinc / metabolism
Substances
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Phenanthrolines
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Recombinant Proteins
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Glutamate-tRNA Ligase
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Zinc
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1,10-phenanthroline