Influence of biphasic calcium phosphate granulometry on bone ingrowth, ceramic resorption, and inflammatory reactions: preliminary in vitro and in vivo study

J Biomed Mater Res. 1999 Jul;46(1):103-11. doi: 10.1002/(sici)1097-4636(199907)46:1<103::aid-jbm12>3.0.co;2-z.

Abstract

Calcium-phosphate ceramics used in surgery, as bone-bonding materials, are currently available in different forms (blocks, granules, etc.). However, progress in noninvasive surgery has favored the development of injectable composite materials associating a polymeric and a dusty mineral phase. The purpose of this study was the in vivo evaluation of biphasic calcium phosphate of various grains sizes, to elucidate the role of granulometries in ceramic degradation/resorption, bone ingrowth, and inflammatory reactions. Three particle sizes were compared: 10-20, 80-100, and 200-400 microm. The 10-20-microm powders provided the best bone ingrowth, with a higher resorption/degradation rate in conjunction with stronger early inflammatory reactions. The 200-400-microm powders showed higher bone ingrowth than 80-100-microm ones, indicating that properties of cell recruitment for osseous apposition and mechanical support for bone bonding may both play a role in both ingrowth mechanisms. Our results suggest that the strong inflammatory reaction in 10-20-microm granulated powders was due to a faster reversal of the resorption/apposition sequence in bone. This may have resulted from massive release of bone ingrowth factors, which implies that the brief inflammatory process observed in the early stages of implantation was favorable to the osteoconduction process.

MeSH terms

  • Bone Development / physiology*
  • Bone Substitutes / adverse effects*
  • Bone Substitutes / chemistry*
  • Calcium Phosphates / adverse effects*
  • Calcium Phosphates / chemistry*
  • Cells, Cultured
  • Ceramics / adverse effects*
  • Ceramics / chemistry*
  • Chemical Phenomena
  • Chemistry, Physical
  • Hydroxyapatites / chemistry
  • Inflammation / chemically induced*
  • Inflammation / pathology
  • Microscopy, Electron, Scanning
  • Particle Size
  • Powders

Substances

  • Bone Substitutes
  • Calcium Phosphates
  • Hydroxyapatites
  • Powders
  • alpha-tricalcium phosphate
  • tetracalcium phosphate
  • calcium phosphate, monobasic, anhydrous
  • calcium phosphate
  • calcium phosphate, dibasic, anhydrous