Ligand recognition in multiallelic pheromone receptors from the basidiomycete Schizophyllum commune studied in yeast

Fungal Genet Biol. 1999 Apr;26(3):190-7. doi: 10.1006/fgbi.1999.1119.

Abstract

The homobasidiomycete Schizophyllum commune encodes a multiallelic pheromone receptor system that distinguishes more than 20 nonself from at least 2 self pheromones. The well-investigated pheromone response system of the yeast Saccharomyces cerevisiae was used to link the FUS1::lacZ reporter system to the heterologous pheromone receptors from S. commune. To investigate yeast G-protein binding, the unchanged heterologous receptor was compared to constructs carrying an exchange of the 3rd cytoplasmatic loop for the Ste2 sequence. A better coupling could be achieved with the altered constructs. In order to examine activation by single pheromones, an artificial peptide based on the sequence of a new putative pheromone gene, bap2(1), in the Balpha2 mating-type locus encoding the shortest pheromone found so far in fungal mating types was used. Thus, we have reassembled the pheromone recognition of the basidiomycete S. commune and constructed a system ideal for specificity analysis in the yeast S. cerevisiae.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chemoreceptor Cells / metabolism*
  • Genes, Fungal*
  • Genes, Mating Type, Fungal*
  • Genes, Reporter / genetics
  • Ligands
  • Molecular Sequence Data
  • Schizophyllum / chemistry
  • Schizophyllum / genetics
  • Schizophyllum / metabolism*
  • Sequence Homology, Nucleic Acid
  • Transfection

Substances

  • Ligands