Abstract
Escherichia coli disruptants defective in the yaeM gene, which is located at 4.2 min on the chromosome map, were constructed and characterized. The disruptants showed auxotrophy for 2-C-methylerythritol, a free alcohol of 2-C-methyl-D-erythritol 4-phosphate that is a biosynthetic precursor in the nonmevalonate pathway. This result clearly shows that the yaeM gene is indeed involved in this pathway in E. coli.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aldose-Ketose Isomerases / biosynthesis*
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Aldose-Ketose Isomerases / genetics*
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Blotting, Southern
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Catalysis
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Cloning, Molecular
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DNA, Fungal / biosynthesis
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DNA, Fungal / genetics
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Erythritol / analogs & derivatives
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Erythritol / metabolism
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Escherichia coli / genetics*
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Escherichia coli / metabolism*
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Genes, Bacterial / genetics*
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Multienzyme Complexes / biosynthesis*
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Multienzyme Complexes / genetics*
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Mutation / genetics
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Mutation / physiology*
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Oxidoreductases / biosynthesis*
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Oxidoreductases / genetics*
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Pentosephosphates / metabolism
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Phenotype
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Sugar Phosphates / metabolism
Substances
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1-deoxylulose 5-phosphate
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2-C-methylerythritol 4-phosphate
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DNA, Fungal
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Multienzyme Complexes
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Pentosephosphates
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Sugar Phosphates
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Oxidoreductases
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1-deoxy-D-xylulose 5-phosphate reductoisomerase
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Aldose-Ketose Isomerases
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Erythritol