Abstract
The Caenorhabditis elegans unc-13, unc-18, and unc-64 genes are required for normal synaptic transmission. The UNC-18 protein binds to the unc-64 gene product C. elegans syntaxin (Ce syntaxin). However, it is not clear how this protein complex is regulated. We show that UNC-13 transiently interacts with the UNC-18-Ce syntaxin complex, resulting in rapid displacement of UNC-18 from the complex. Genetic and biochemical evidence is presented that UNC-13 contributes to the modulation of the interaction between UNC-18 and Ce syntaxin.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alleles
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Animals
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Antigens, Surface / genetics
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Antigens, Surface / metabolism
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Caenorhabditis elegans
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Caenorhabditis elegans Proteins*
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Carrier Proteins*
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Exocytosis / physiology
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Gene Deletion
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Gene Expression / physiology
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Genes, Helminth / physiology
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Helminth Proteins / genetics
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Helminth Proteins / metabolism*
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Membrane Proteins / metabolism*
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Mutagenesis, Insertional / physiology
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Neurons / chemistry
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Neurons / physiology
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Peptide Fragments / metabolism
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Phenotype
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Phosphoproteins*
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Qa-SNARE Proteins
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SNARE Proteins
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Synaptic Transmission / genetics
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Synaptic Vesicles / chemistry
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Synaptic Vesicles / metabolism
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Syntaxin 1
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Vesicular Transport Proteins*
Substances
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Antigens, Surface
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Caenorhabditis elegans Proteins
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Carrier Proteins
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Helminth Proteins
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Membrane Proteins
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Nerve Tissue Proteins
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Peptide Fragments
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Phosphoproteins
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Qa-SNARE Proteins
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SNARE Proteins
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Syntaxin 1
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Unc-18 protein, C elegans
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Vesicular Transport Proteins
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phorbol ester binding protein
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unc-64 protein, C elegans