Abstract
To identify genes regulated by the B mating-type genes a differential RNA display was established for the homobasidiomycete Schizophyllum commune and a gene, brt1, was identified. The mRNA concerned is highly abundant in monokaryotic mycelia and is down-regulated in matings in which either the B genes alone or else both the A and B genes are different between the mates. This places the gene brt1 under the control of the pheromone response system of S. commune encoded by the B mating-type loci. Sequence analysis revealed similarity to a novel protein family, two members of which have been shown to inhibit translational re-initiation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Blotting, Northern
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Chromosome Mapping
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Cloning, Molecular
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Crosses, Genetic
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Deoxyribonuclease BamHI / genetics
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Down-Regulation
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism
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Gene Deletion
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Gene Expression Regulation, Fungal*
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Genetic Markers
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Genetic Techniques
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In Situ Hybridization / methods
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Molecular Sequence Data
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RNA, Fungal
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Reproduction / genetics
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Schizophyllum / genetics*
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Schizophyllum / growth & development
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Sequence Homology, Amino Acid
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Transcription, Genetic
Substances
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BRT1 protein, Schizophyllum commune
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Fungal Proteins
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Genetic Markers
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RNA, Fungal
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Deoxyribonuclease BamHI