Abstract
Effectiveness of electrochemotherapy against colorectal carcinoma (CRC) was investigated in vitro using murine CRC cell lines. Electropermeabilization did not increase the sensitivity of CRC cells to 5-fluorouracil or cisplatin. Conversely, electropermeabilization markedly increased the sensitivity of CRC cells to bleomycin (BLM), resulting in 1,000-fold higher susceptibility. Subsequent analyses revealed that electropermeabilization significantly increased intracellular BLM levels of CRC cells. These results suggest that electrochemotherapy with BLM is a promising modality for the treatment of CRC, and that electrochemotherapy can be translated from the treatment of superficial tumors to the treatment of internal tumors including CRC.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / chemically induced
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Adenocarcinoma / drug therapy*
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Adenocarcinoma / epidemiology
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Adenocarcinoma / pathology
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Animals
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Antineoplastic Agents / administration & dosage*
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Antineoplastic Agents / pharmacology
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Antineoplastic Agents / therapeutic use
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Bleomycin / administration & dosage*
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Bleomycin / pharmacology
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Bleomycin / therapeutic use
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Cell Membrane Permeability
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Cisplatin / administration & dosage*
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Cisplatin / pharmacology
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Cisplatin / therapeutic use
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Colorectal Neoplasms / chemically induced
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Colorectal Neoplasms / drug therapy*
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Colorectal Neoplasms / epidemiology
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Colorectal Neoplasms / pathology
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Culture Media, Serum-Free
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Dimethylhydrazines
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Drug Screening Assays, Antitumor
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Electroporation*
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Fluorouracil / administration & dosage*
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Fluorouracil / pharmacology
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Fluorouracil / therapeutic use
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Humans
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Intracellular Fluid / chemistry
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Tumor Cells, Cultured / drug effects
Substances
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Antineoplastic Agents
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Culture Media, Serum-Free
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Dimethylhydrazines
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Bleomycin
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Cisplatin
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Fluorouracil