Identification of CARE-2-negative Candida albicans isolates as Candida dubliniensis

Mycoses. 1999 Apr;42(1-2):29-32. doi: 10.1046/j.1439-0507.1999.00259.x.

Abstract

Among 302 clinical yeast isolates originally identified as Candida albicans, we found 16 isolates that did not hybridize with the C. albicans-specific repetitive DNA element CARE-2. These 16 isolates produced abundant chlamydospores, did not grow at 43 degrees C, and exhibited a distinct randomly amplified polymorphic DNA profile. Sequence analysis of part of the 28S rDNA demonstrated that the CARE-2-negative isolates are not an atypical subgroup of C. albicans but belong to the recently described new species C. dubliniensis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Candida / classification*
  • Candida / genetics*
  • Candida / isolation & purification
  • Candida albicans / classification*
  • Candida albicans / genetics
  • Candidiasis / microbiology*
  • DNA, Fungal / genetics
  • DNA, Ribosomal / genetics
  • Humans
  • Mycological Typing Techniques*
  • Phylogeny
  • RNA, Ribosomal, 28S / genetics
  • Random Amplified Polymorphic DNA Technique
  • Repetitive Sequences, Nucleic Acid

Substances

  • DNA, Fungal
  • DNA, Ribosomal
  • RNA, Ribosomal, 28S