Use of polymerase chain reaction for citrate synthase gene to diagnose Bartonella quintana endocarditis

Am J Clin Pathol. 1999 Jul;112(1):36-40. doi: 10.1093/ajcp/112.1.36.

Abstract

We describe aortic valve endocarditis caused by Bartonella quintana in a 31-year-old man. The diagnosis was made on the basis of polymerase chain reaction amplification of the B quintana citrate synthase gene from cardiac valve tissue, the compatibility of histochemical stains of cardiac valve tissue, and serologic studies.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Antigens, Bacterial / analysis
  • Aortic Valve / microbiology*
  • Aortic Valve / pathology
  • Bartonella quintana / enzymology*
  • Bartonella quintana / immunology
  • Bartonella quintana / isolation & purification
  • Citrate (si)-Synthase / genetics*
  • DNA Primers / chemistry
  • DNA, Bacterial / analysis
  • Endocarditis, Bacterial / diagnosis
  • Endocarditis, Bacterial / microbiology*
  • HIV Infections / complications
  • Heart Valve Diseases / diagnosis
  • Heart Valve Diseases / microbiology*
  • Humans
  • Immunoglobulin G / analysis
  • Male
  • Polymerase Chain Reaction / methods
  • Trench Fever / diagnosis
  • Trench Fever / microbiology*

Substances

  • Antigens, Bacterial
  • DNA Primers
  • DNA, Bacterial
  • Immunoglobulin G
  • Citrate (si)-Synthase