Photoinduced cross-linking of RNA by cis-Rh(phen)2Cl2+ and cis-Rh(phen)(phi)Cl2+: a new family of light activatable nucleic acid cross-linking agents

Bioorg Med Chem Lett. 1999 Jun 21;9(12):1703-8. doi: 10.1016/s0960-894x(99)00265-6.

Abstract

The metal complexes, cis-Rh(phen)2Cl2+ and its more hydrophobic analog cis-Rh(phen)(phi)Cl2+, have been shown to photocross-link the 120-base phi29-encoded pRNA. Primer extension on the cis-Rh(phen)(phi)Cl2(+)-photocross-linked RNA revealed that guanines are responsible for the interstrand cross-links.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacillus Phages / genetics
  • Base Sequence
  • Cross-Linking Reagents / chemistry*
  • Guanine / chemistry
  • Light
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Organometallic Compounds / chemistry*
  • Phenanthrenes / chemistry*
  • Phenanthrolines / chemistry*
  • Photochemistry
  • RNA, Viral / chemistry*

Substances

  • (1,10-phenanthroline)(9,10-phenanthrenequinonediimine)rhodamine(III)
  • Cross-Linking Reagents
  • Organometallic Compounds
  • Phenanthrenes
  • Phenanthrolines
  • RNA, Viral
  • bis(1,10-phenanthroline)rhodium(III)
  • Guanine