Abstract
An adenovirus vector encoding the human Bcl-2 gene (hBcl-2) was derived. In vivo expression of hBcl-2 in murine livers enhanced and prolonged adenovirus-mediated gene expression. Furthermore, in the hBcl-2-treated group a significant reduction in the apoptosis induced by the adenovirus vector was observed. Thus, the cytoprotection of the vector-infected cells with antiapoptotic genes appears promising for successful in vivo gene therapy.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviruses, Human* / physiology
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Animals
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Apoptosis*
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Aspartate Aminotransferases / metabolism
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Cytokines / metabolism
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Gene Expression Regulation*
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Genetic Vectors*
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HeLa Cells
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Humans
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Liver / pathology
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Liver / virology
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Male
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Mice
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Mice, Inbred C57BL
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Proto-Oncogene Proteins c-bcl-2 / genetics
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Proto-Oncogene Proteins c-bcl-2 / metabolism*
Substances
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Cytokines
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Proto-Oncogene Proteins c-bcl-2
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Aspartate Aminotransferases