Leginon: a system for fully automated acquisition of 1000 electron micrographs a day

Ultramicroscopy. 1999 Jul;77(3-4):153-61. doi: 10.1016/s0304-3991(99)00043-1.

Abstract

We have developed a system to automatically acquire large numbers of acceptable quality images from specimens of negatively stained catalase, a biological protein which forms crystals. In this paper we will describe the details of the system architecture and analyze the performance of the system as compared to a human operator. The ultimate goal of the system if to automate the process of acquiring cryo-electron micrographs.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Automation / methods*
  • Humans
  • Microscopy, Electron / methods*