Steroid-DNA conjugates: improved triplex formation with 5-amido-(7-deoxycholic acid)-dU incorporated oligonucleotides

Bioorg Med Chem Lett. 1999 Jul 5;9(13):1789-94. doi: 10.1016/s0960-894x(99)00274-7.

Abstract

The synthesis of oligodeoxyribonucleotides incorporating 7-deoxycholic acid conjugated at C5 of dU is described. When used as third strand, these form oligonucleotide triplexes with higher stability compared to unmodified controls at physiological pH.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA / chemistry*
  • DNA / metabolism*
  • Dose-Response Relationship, Radiation
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Oligonucleotides / chemistry*
  • Oligonucleotides / radiation effects
  • Steroids / chemistry*
  • Steroids / metabolism*
  • Temperature
  • Ultraviolet Rays
  • Uracil / chemistry

Substances

  • Oligonucleotides
  • Steroids
  • Uracil
  • DNA