Crystallization and preliminary X-ray diffraction analysis of a myotoxic phospholipase A(2) homologue from Bothrops neuwiedi pauloensis venom

M R Fontes; A M Soares; V M Rodrigues; A C Fernandes; R J Da Silva; J R Giglio

doi:10.1016/s0167-4838(99)00120-x

Crystallization and preliminary X-ray diffraction analysis of a myotoxic phospholipase A(2) homologue from Bothrops neuwiedi pauloensis venom

Biochim Biophys Acta. 1999 Jul 13;1432(2):393-5. doi: 10.1016/s0167-4838(99)00120-x.

Authors

M R Fontes¹, A M Soares, V M Rodrigues, A C Fernandes, R J Da Silva, J R Giglio

Affiliation

¹ Departamento de Física e Biofísica, Instituto de Biociências, UNESP, C.P. 510, CEP 18618-000, Botucatu-SP, Brazil. [email protected]

PMID: 10407160
DOI: 10.1016/s0167-4838(99)00120-x

Abstract

Crystals of a myotoxic phospholipase A(2) from Bothrops neuwiedi pauloensis have been obtained. They diffracted at 2.5 A resolution using a synchrotron radiation source and belong to space group P3(1)21. Preliminary analysis shows that there are two molecules in the asymmetric unit.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bothrops*
Brazil
Crotalid Venoms / chemistry*
Crystallization
Neurotoxins / chemistry*
Phospholipases A / chemistry*
Reptilian Proteins
X-Ray Diffraction

Substances

Crotalid Venoms
Neurotoxins
Reptilian Proteins
myotoxic component of rattlesnake venom
myotoxin II, Bothrops moojeni
Phospholipases A