A method for characterizing longitudinal chromatic aberration of microscope objectives using a confocal optical system

J Microsc. 1999 Jul:195 (Pt 1):17-22. doi: 10.1046/j.1365-2818.1999.00488.x.

Abstract

We describe a novel method of characterizing the longitudinal chromatic aberration of microscope objectives by recording a series of axial responses as a function of wavelength as a plane reflector is scanned through the focal region of a confocal microscope. Measurements are presented for a variety of objectives with differing degrees of correction. The use of the chromatic focal shift to measure surface profiles is also discussed.