Identification of genes differentially expressed in TLS-CHOP carrying myxoid liposarcomas

Int J Cancer. 1999 Sep 24;83(1):30-3. doi: 10.1002/(sici)1097-0215(19990924)83:1<30::aid-ijc6>3.0.co;2-4.

Abstract

Myxoid liposarcomas (MLS) carry a t(12;16) or, more rarely, a t(12;22) resulting in fusion of the transcription factor gene CHOP on chromosome 12 with TLS/FUS on chromosome 16 or EWS on chromosome 22. The chimeric TLS-CHOP or EWS-CHOP proteins most probably function as abnormal transcription factors, causing transcriptional de-regulation of several target genes and relaxation of functions critical for growth and differentiation control. A PCR-based subtractive hybridization technique was used to identify genes that are differentially expressed in TLS-CHOP-carrying MLS but not in normal fat tissue. Six myxoid-liposarcoma-associated transcripts, MLAT, were isolated. The genes identified as MLAT can be divided into 2 groups. MLAT1, 2 and 6 show high similarity to glia-derived nexin, neuronatin and the RET oncogene, respectively, all normally involved in development of tissues of neural origin. MLAT3 to MLAT5 represent new genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CCAAT-Enhancer-Binding Proteins*
  • Carrier Proteins / genetics
  • Cells, Cultured
  • DNA, Complementary / genetics
  • DNA, Neoplasm / genetics
  • Drosophila Proteins*
  • Fibroblasts
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Liposarcoma, Myxoid / genetics*
  • Membrane Proteins / genetics
  • Nerve Tissue Proteins / genetics
  • Nuclear Proteins / genetics*
  • Oncogene Proteins, Fusion / genetics*
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins c-ret
  • RNA-Binding Protein FUS*
  • Receptor Protein-Tyrosine Kinases / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription Factor CHOP
  • Transfection
  • Tumor Cells, Cultured

Substances

  • CCAAT-Enhancer-Binding Proteins
  • Carrier Proteins
  • DNA, Complementary
  • DNA, Neoplasm
  • Drosophila Proteins
  • Membrane Proteins
  • NNAT protein, human
  • Nerve Tissue Proteins
  • Nuclear Proteins
  • Oncogene Proteins, Fusion
  • Proto-Oncogene Proteins
  • RNA-Binding Protein FUS
  • TLS-CHOP fusion protein, human
  • Transcription Factor CHOP
  • Proto-Oncogene Proteins c-ret
  • Receptor Protein-Tyrosine Kinases
  • Ret protein, Drosophila