Rapid, high-sensitivity imaging of radiolabeled gels with microchannel plate detectors

J E Lees; P G Richards

doi:10.1002/(SICI)1522-2683(19990701)20:10<2139::AID-ELPS2139>3.0.CO;2-R

Rapid, high-sensitivity imaging of radiolabeled gels with microchannel plate detectors

Electrophoresis. 1999 Jul;20(10):2139-43. doi: 10.1002/(SICI)1522-2683(19990701)20:10<2139::AID-ELPS2139>3.0.CO;2-R.

Authors

J E Lees¹, P G Richards

Affiliation

¹ Space Research Centre, University of Leicester, UK. [email protected]

Abstract

A microchannel plate detector has been used to image tritium-labeled protein on one- and two-dimensional electrophoresis gels. The good spatial resolution (70 microns) and high sensitivity (6.0 dpm/mm2) of the imaging system allows detection of low levels (femto moles) of labelled proteins. We are currently using the detector for identification of new targets involved in organophosphate neurotoxicity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain Chemistry
Electrophoresis*
Electrophoresis, Gel, Two-Dimensional*
Electrophoresis, Polyacrylamide Gel
Image Processing, Computer-Assisted / methods*
Isoelectric Focusing
Isoflurophate / toxicity
Male
Nerve Tissue Proteins / analysis*
Nervous System Diseases / chemically induced
Organothiophosphorus Compounds / toxicity
Rats
Sensitivity and Specificity
Tritium*

Substances

Nerve Tissue Proteins
Organothiophosphorus Compounds
Tritium
Isoflurophate