Biodistribution studies of the 186Re complex of 3-amino-1-hydroxypropylidene-1,1-bisphosphonic acid in mice

Nucl Med Commun. 1999 Jun;20(6):551-7. doi: 10.1097/00006231-199906000-00010.

Abstract

Preliminary studies of 186Re-labelled 3-amino-1-hydroxypropylidene-1, 1-bisphosphonic acid (APD) were performed to determine its potential for bone pain palliation, and as a treatment for increased bone resorption. The synthesis of 186Re-APD was carried out by reduction of 186Re-perrhenate in the presence of SnCl2. The APD kit, comprising 2.5 mg of APD, 2.5 mg of gentisic acid and 1 mg of Sn++ as SnCl2 2H2O, was prepared in-house. The APD was labelled with 186Re and injected intravenously into normal mice. Mice were subsequently sacrificed at 1, 3, 24, 48, 72, 168 and 240 h post-injection. The greatest accumulation of 186Re-labelled APD was found in bone, resulting in bone-to-blood ratios of 25, 35, 65, 100, 151, 181 and 189, respectively. 186Re-APD showed high uptake in bone, and relatively low uptake in soft tissue, suggesting that 186Re-APD is a potential agent for bone therapy.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Bone and Bones / diagnostic imaging
  • Diphosphonates / pharmacokinetics*
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Pamidronate
  • Radioisotopes / pharmacokinetics*
  • Radionuclide Imaging
  • Rhenium / pharmacokinetics*
  • Tissue Distribution

Substances

  • Diphosphonates
  • Radioisotopes
  • Rhenium
  • Pamidronate