Synthesis of phosphono analogues of dihydroxyacetone phosphate and glyceraldehyde 3-phosphate

Bioorg Med Chem. 1999 Jul;7(7):1403-12. doi: 10.1016/s0968-0896(99)00065-6.

Abstract

The present paper describes the synthetic routes of six phosphono analogues of dihydroxyacetone phosphate and five phosphono analogues of glyceraldehyde 3-phosphate through alpha-, beta- and gamma-hydroxyphosphonate esters precursors containing a protected carbonyl group. In some situations, depending on the sequence used for the deprotection of the phosphonate and carbonyl groups, the aldol/ketol rearrangement allowed the synthesis of either dihydroxyacetone phosphate or glyceraldehyde 3-phosphate analogues from the same precursors. All these analogues are of interest both as active-site probes and as potential substrates for glycolytic enzymes such as fructose 1,6-diphosphate aldolases (EC 4.1.2.13).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dihydroxyacetone Phosphate / analogs & derivatives*
  • Dihydroxyacetone Phosphate / chemistry*
  • Dihydroxyacetone Phosphate / metabolism
  • Fructose-Bisphosphate Aldolase / metabolism
  • Glyceraldehyde 3-Phosphate / analogs & derivatives*
  • Glyceraldehyde 3-Phosphate / chemistry*
  • Glyceraldehyde 3-Phosphate / metabolism
  • Muscle, Skeletal / enzymology
  • Organophosphonates / chemistry
  • Rabbits
  • Substrate Specificity

Substances

  • Organophosphonates
  • Glyceraldehyde 3-Phosphate
  • Dihydroxyacetone Phosphate
  • Fructose-Bisphosphate Aldolase