Abstract
Transforming growth factor-beta (TGF-beta) superfamily members constitute a group of multifunctional factors that are able to stimulate apoptotic cell death in a variety of cells. In this report, we show that a zinc-finger transcription factor (TIEG) is an immediate early gene transcriptionally induced by TGF-beta in the epithelial Mv1Lu cell line. We also demonstrate that, mimicking TGF-beta effects, ectopic overexpression of TIEG is sufficient to trigger the apoptotic cell program in these cells, which is preceded by a decrease of Bcl-2 protein levels. Finally, apoptotic events elicited by TIEG overexpression can be effectively prevented by ectopic co-expression of Bcl-2. On the basis of these results we suggest that induction of TIEG expression has a role in the pro-apoptotic properties of TGF-beta.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis / physiology*
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Cell Division / genetics
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Cell Line / drug effects
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Cycloheximide / pharmacology
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DNA Fragmentation
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Dactinomycin / pharmacology
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Epithelium / metabolism
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Epithelium / pathology
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Genes, Immediate-Early
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Lung / cytology*
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Lung / metabolism
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Lung / pathology
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Mice
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Nucleic Acid Synthesis Inhibitors / pharmacology
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Protein Synthesis Inhibitors / pharmacology
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Proto-Oncogene Proteins c-bcl-2 / genetics
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Proto-Oncogene Proteins c-bcl-2 / metabolism
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Proto-Oncogene Proteins c-jun / genetics
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Proto-Oncogene Proteins c-jun / metabolism
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RNA, Messenger
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription, Genetic
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Transforming Growth Factor beta / metabolism*
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Transforming Growth Factor beta / pharmacology
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Zinc Fingers / genetics*
Substances
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DNA-Binding Proteins
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Nucleic Acid Synthesis Inhibitors
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Protein Synthesis Inhibitors
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Proto-Oncogene Proteins c-bcl-2
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Proto-Oncogene Proteins c-jun
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RNA, Messenger
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Recombinant Proteins
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Tieg1 protein, mouse
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Transcription Factors
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Transforming Growth Factor beta
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Dactinomycin
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Cycloheximide