Abstract
Overexpression of the isopentenyltransferase gene (ipt) from the Ti-plasmid of Agrobacterium tumefaciens increases cytokinin levels, leading to generation of shoots from transformed plant cells. When combined with a dexamethasone-inducible system for controlling expression, ipt expression can be used to select for transgenic regenerants without using an antibiotic-resistance marker. The combined system allows efficient cointroduction of multiple genes (in addition to ipt) and produces transgenic plants without morphological or developmental defects.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Agrobacterium tumefaciens / genetics
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Alkyl and Aryl Transferases / biosynthesis
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Alkyl and Aryl Transferases / genetics*
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Cytokinins / biosynthesis
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Dexamethasone / pharmacology
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Enzyme Induction
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Genes, Bacterial
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Genetic Markers*
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Lactuca / genetics
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Luciferases / genetics
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Magnoliopsida / genetics*
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Nicotiana / genetics
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Phosphotransferases (Alcohol Group Acceptor) / genetics
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Plant Roots / growth & development
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Plant Shoots / growth & development
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Plants, Genetically Modified
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Plants, Toxic
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Plasmids
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Selection, Genetic
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Transformation, Genetic*
Substances
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Cytokinins
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Genetic Markers
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Dexamethasone
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Luciferases
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Alkyl and Aryl Transferases
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adenylate isopentenyltransferase
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Phosphotransferases (Alcohol Group Acceptor)
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hygromycin-B kinase