Inducible isopentenyl transferase as a high-efficiency marker for plant transformation

Nat Biotechnol. 1999 Sep;17(9):916-9. doi: 10.1038/12914.

Abstract

Overexpression of the isopentenyltransferase gene (ipt) from the Ti-plasmid of Agrobacterium tumefaciens increases cytokinin levels, leading to generation of shoots from transformed plant cells. When combined with a dexamethasone-inducible system for controlling expression, ipt expression can be used to select for transgenic regenerants without using an antibiotic-resistance marker. The combined system allows efficient cointroduction of multiple genes (in addition to ipt) and produces transgenic plants without morphological or developmental defects.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agrobacterium tumefaciens / genetics
  • Alkyl and Aryl Transferases / biosynthesis
  • Alkyl and Aryl Transferases / genetics*
  • Cytokinins / biosynthesis
  • Dexamethasone / pharmacology
  • Enzyme Induction
  • Genes, Bacterial
  • Genetic Markers*
  • Lactuca / genetics
  • Luciferases / genetics
  • Magnoliopsida / genetics*
  • Nicotiana / genetics
  • Phosphotransferases (Alcohol Group Acceptor) / genetics
  • Plant Roots / growth & development
  • Plant Shoots / growth & development
  • Plants, Genetically Modified
  • Plants, Toxic
  • Plasmids
  • Selection, Genetic
  • Transformation, Genetic*

Substances

  • Cytokinins
  • Genetic Markers
  • Dexamethasone
  • Luciferases
  • Alkyl and Aryl Transferases
  • adenylate isopentenyltransferase
  • Phosphotransferases (Alcohol Group Acceptor)
  • hygromycin-B kinase