Abstract
Immunohistochemical analysis of the expression of the autosomal apoptosis regulator genes, BAX, bcl-2, p53, and cyclic-AMP responsive element modulator (CREM) in testis biopsies from infertile men demonstrated that BAX, bcl-2, and p53 immunoreactivity was absent irrespective of seminiferous tubule histology. In contrast, cell-specific CREM immunoreactivity in round spermatids, with complete absence of CREM in patient biopsies showing spermatocyte maturation arrest and Sertoli cell only, was evident, suggesting a possible role of autosomal genes in the regulation of apoptosis in human spermatogenesis regulation.
MeSH terms
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Adult
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Apoptosis / genetics*
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Cyclic AMP Response Element Modulator
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DNA-Binding Proteins / metabolism*
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Humans
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Immunohistochemistry
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Male
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Oligospermia / metabolism
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Oligospermia / pathology
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Proto-Oncogene Proteins / metabolism*
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Proto-Oncogene Proteins c-bcl-2 / metabolism*
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Repressor Proteins*
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Sertoli Cells / cytology
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Sertoli Cells / metabolism
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Spermatids / cytology
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Spermatids / metabolism
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Spermatocytes / cytology
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Spermatocytes / metabolism
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Spermatogenesis / genetics*
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Tumor Suppressor Protein p53 / metabolism*
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bcl-2-Associated X Protein
Substances
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BAX protein, human
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DNA-Binding Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-2
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Repressor Proteins
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Tumor Suppressor Protein p53
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bcl-2-Associated X Protein
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Cyclic AMP Response Element Modulator