Abstract
The complete genomic sequencing of Methanococcus jannaschii cannot identify the gene for the cysteine-specific member of aminoacyl-tRNA synthetases. However, we show here that enzyme activity is present in the cell lysate of M. jannaschii. The demonstration of this activity suggests a direct pathway for the synthesis of cysteinyl-tRNA(Cys) during protein synthesis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acyl-tRNA Synthetases / genetics*
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Amino Acyl-tRNA Synthetases / isolation & purification
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Amino Acyl-tRNA Synthetases / metabolism*
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Genes, Archaeal*
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Genome
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Kinetics
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Methanococcus / enzymology*
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Methanococcus / genetics*
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Thermodynamics
Substances
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Amino Acyl-tRNA Synthetases
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cysteinyl-tRNA synthetase