Human Dmc1 protein binds DNA as an octameric ring

Proc Natl Acad Sci U S A. 1999 Sep 14;96(19):10684-8. doi: 10.1073/pnas.96.19.10684.

Abstract

The bacterial RecA protein has been the most intensively studied enzyme in homologous genetic recombination. The core of RecA is structurally homologous to that of the F1-ATPase and helicases. Like the F1-ATPase and ring helicases, RecA forms a hexameric ring. The human Dmc1 (hDmc1) protein, a meiosis-specific recombinase, is homologous to RecA. We show that hDmc1 forms octameric rings. Unlike RecA and Rad51, however, hDmc1 protein does not form helical filaments. The hDmc1 ring binds DNA in the central channel, as do the ring helicases, which is likely to represent the active form of the protein. These observations indicate that the conservation of the RecA-like ring structure extends from bacteria to humans, and that some RecA homologs may form both rings and filaments, whereas others may function only as rings.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacteriophages / metabolism
  • Cell Cycle Proteins*
  • DNA / metabolism*
  • DNA, Single-Stranded / metabolism
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / metabolism*
  • Escherichia coli / genetics
  • Humans
  • Microscopy, Electron
  • Protein Conformation
  • Rec A Recombinases / chemistry
  • Rec A Recombinases / metabolism

Substances

  • Cell Cycle Proteins
  • DNA, Single-Stranded
  • DNA-Binding Proteins
  • DNA
  • Rec A Recombinases
  • DMC1 protein, human