Abstract
We generated transgenic mice expressing a single-chain beta2-microglobulin (beta2m)-H-2Dd. The cell-surface beta2m-H-2Dd molecule was expressed on a beta2m-deficient background and reacted with appropriate mAbs. It was of the expected m.w. and directed the normal development of CD8+ T cells in the thymus of a broad TCR repertoire. It also presented both exogenously provided and endogenous peptide Ags to effector CD8+ T cells. In tests of NK cell education and function, it failed to reveal any interaction with NK cells, suggesting that the site of the interaction of NK receptors with H-2Dd was disrupted. Thus, the sites of TCR and NK receptor interaction with H-2Dd are distinct, an observation consistent with independent modes of TCR and NK receptor evolution and function.
MeSH terms
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Animals
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Antigens, Ly*
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CD8-Positive T-Lymphocytes / cytology
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CD8-Positive T-Lymphocytes / immunology
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CD8-Positive T-Lymphocytes / metabolism
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CD8-Positive T-Lymphocytes / virology
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Cell Differentiation / genetics
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Cell Differentiation / immunology
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Cytotoxicity, Immunologic / genetics
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Cytotoxicity, Immunologic / immunology
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H-2 Antigens / biosynthesis
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H-2 Antigens / genetics
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H-2 Antigens / metabolism*
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Killer Cells, Natural / immunology
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Killer Cells, Natural / metabolism*
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Lectins, C-Type
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Membrane Glycoproteins / metabolism
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mice, Knockout
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Mice, Transgenic
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Protein Binding / genetics
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Protein Binding / immunology
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Receptors, Antigen, T-Cell / genetics
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Receptors, Antigen, T-Cell / metabolism*
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Receptors, Immunologic / genetics
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Receptors, Immunologic / metabolism*
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Receptors, NK Cell Lectin-Like
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T-Lymphocytes, Cytotoxic / immunology
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T-Lymphocytes, Cytotoxic / metabolism*
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T-Lymphocytes, Cytotoxic / virology
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Transgenes / immunology
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Vaccinia virus / immunology
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beta 2-Microglobulin / deficiency
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beta 2-Microglobulin / genetics
Substances
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Antigens, Ly
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H-2 Antigens
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H-2K(K) antigen
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Lectins, C-Type
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Membrane Glycoproteins
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Receptors, Antigen, T-Cell
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Receptors, Immunologic
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Receptors, NK Cell Lectin-Like
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beta 2-Microglobulin