Abstract
From a human placenta cDNA library, we isolated a novel gene whose predicted product is highly homologous in amino acid sequence to human gadd45 and murine MyD118 proteins (about 55% and 52% identity, respectively). The cDNA clone, designated GRP17 (gadd-related protein, 17 kDa), contained a 477-bp open reading frame encoding 159 amino acids. Northern blot analysis revealed strong expression of a 1.35-kb transcript in heart, placenta, liver, skeletal muscle, prostate, testis, and ovary. A 1.7-kb additional transcript was detected in liver. We assigned the GRP17 gene to chromosome 9q22.1-q22.2 by fluorescence in situ hybridization (FISH).
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Differentiation / biosynthesis
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Antigens, Differentiation / chemistry
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Antigens, Differentiation / genetics*
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Base Sequence
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Carrier Proteins*
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Chromosome Mapping
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Chromosomes, Human, Pair 9*
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Cloning, Molecular
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DNA Damage
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DNA, Complementary / genetics
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Female
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GADD45 Proteins
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Humans
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In Situ Hybridization, Fluorescence
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Intracellular Signaling Peptides and Proteins*
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Male
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Mice
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Molecular Sequence Data
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Organ Specificity
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Pregnancy
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Proteins / chemistry
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Proteins / genetics*
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transcription, Genetic
Substances
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Antigens, Differentiation
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Carrier Proteins
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DNA, Complementary
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GADD45B protein, human
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GADD45G protein, human
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Gadd45b protein, mouse
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Intracellular Signaling Peptides and Proteins
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Proteins
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Recombinant Proteins