Abstract
We present a simple and inexpensive method for producing 15N-labeled Saccharomyces cerevisiae iso-1-cytochrome c in Escherichia coli. The labeled protein gives excellent NMR spectra.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Cytochrome c Group / biosynthesis*
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Escherichia coli / enzymology*
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Magnetic Resonance Spectroscopy
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Nitrogen Isotopes
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Saccharomyces cerevisiae / enzymology
Substances
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Cytochrome c Group
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Nitrogen Isotopes