Structure of the murine TRAF1 gene

Mol Immunol. 1999 Jun;36(9):611-7. doi: 10.1016/s0161-5890(99)00075-9.

Abstract

We have cloned, characterized and sequenced the murine TNF Receptor Associated Factor 1 (TRAF1) gene. Restriction mapping and Southern blotting analysis revealed that the TRAF1 gene comprises 10 exons and 9 intervening introns and spreads over 18 kb of genomic DNA. 5'-RACE analysis of the TRAF1 transcript using mRNA from activated spleen B cells revealed several transcription start sites between positions -42 to +4 relative to the 5'end of the murine TRAF1 cDNA sequence. We also isolated and sequenced the 5'-upstream promoter region, which lacks TATA-like and CAAT-like sites but contains GC-rich sequences. Taken together, these results suggest that the TRAF1 gene promoter is a member of the class of Sp-1-dependent promoters. Near the transcription initiation start site we identified three identical decanucleotide repeats (CCAGCCCAGC) which may play a role in the transcriptional regulation of TRAF1 expression. In addition we show that TRAF1 mRNA is not expressed in non-stimulated lymphocytes but can be induced upon activation with different stimuli, including anti-CD3, anti-IgM, anti-CD40 antibodies, LPS, or a combination of phorbol-12-myristate-13-acetate and ionomycin.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes
  • Base Sequence
  • Cloning, Molecular
  • Exons
  • Gene Expression
  • Gene Expression Regulation
  • Introns
  • Lymphocyte Activation
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Proteins / genetics*
  • Receptors, Tumor Necrosis Factor*
  • Restriction Mapping
  • T-Lymphocytes
  • TNF Receptor-Associated Factor 1
  • Tissue Distribution
  • Transcription, Genetic

Substances

  • Proteins
  • Receptors, Tumor Necrosis Factor
  • TNF Receptor-Associated Factor 1